E-ISSN 2250-0944 | ISSN 2250-1150
 
2023, Vol: 13, Issue: 2
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Research Article 


Novel Anlytical Rp-Hplc Method for Simultaneous Determination of Antiretroviral Drugs

USHA KONDLA, VANKUDOTH KAVITHA,GOPI SWAPNA,PITTA LAVANYA.

Abstract
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the simultaneousestimation of cobicistat and elvitegravir in the pharmaceutical dosage form. A column of ODS (250mm 4.6mm; i.d and 5µ particle size) was used along with the mobile phase comprising of 0.02M dipotassium hydrogen orthophosphate buffer (pH adjusted to 3.3) and methanol in the ratio of 80:20 (v/v). The flow rate was maintained at 1.0 ml/min and the effluents monitored at 254 nm. The retention time for cobicistat was found to be 2.58 ± 0.3 min and elvitegravir was 3.71 ± 0.3 min. The detection concentration was linear over 125-750 µg/ml for cobicistat and 12.5-75 µg/ml for elvitegravir. Regression equations of cobicistat and elvitegravir were found to be y = 25883x + 19711 and y = 27696x + 6046 respectively with regression co-efficient 0.999. The % RSD for Intra and Inter day precision was < 2%. The accuracy of method was validated by recovery studies and found to be significant within acceptable range 98-102%. The developed method was successfully validated in accordance with ICH guidelines.

Key words: Cobicistat, Elvitegravir, Anti-HIV agent, CYP3A Inhibitors, Validation, ICH guidelines


 
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How to Cite this Article
Pubmed Style

USHA KONDLA, VANKUDOTH KAVITHA,GOPI SWAPNA,PITTA LAVANYA. Novel Anlytical Rp-Hplc Method for Simultaneous Determination of Antiretroviral Drugs. IJPRT. Online First: 23 Sep, 2022. doi:10.31838/ijprt/13.01.11

Web Style

USHA KONDLA, VANKUDOTH KAVITHA,GOPI SWAPNA,PITTA LAVANYA. Novel Anlytical Rp-Hplc Method for Simultaneous Determination of Antiretroviral Drugs. https://www.ijprt.org/?mno=117137 [Access: March 03, 2023]. doi:10.31838/ijprt/13.01.11

AMA (American Medical Association) Style

USHA KONDLA, VANKUDOTH KAVITHA,GOPI SWAPNA,PITTA LAVANYA. Novel Anlytical Rp-Hplc Method for Simultaneous Determination of Antiretroviral Drugs. IJPRT. Online First: 23 Sep, 2022. doi:10.31838/ijprt/13.01.11


Vancouver/ICMJE Style

USHA KONDLA, VANKUDOTH KAVITHA,GOPI SWAPNA,PITTA LAVANYA. Novel Anlytical Rp-Hplc Method for Simultaneous Determination of Antiretroviral Drugs. IJPRT, [cited March 03, 2023]; Online First: 23 Sep, 2022. doi:10.31838/ijprt/13.01.11


Harvard Style

USHA KONDLA, VANKUDOTH KAVITHA,GOPI SWAPNA,PITTA LAVANYA (2022) Novel Anlytical Rp-Hplc Method for Simultaneous Determination of Antiretroviral Drugs. IJPRT, Online First: 23 Sep, 2022. doi:10.31838/ijprt/13.01.11


Turabian Style

USHA KONDLA, VANKUDOTH KAVITHA,GOPI SWAPNA,PITTA LAVANYA. 2022. Novel Anlytical Rp-Hplc Method for Simultaneous Determination of Antiretroviral Drugs. International Journal of Pharmacy Research & Technology, Online First: 23 Sep, 2022. doi:10.31838/ijprt/13.01.11


Chicago Style

USHA KONDLA, VANKUDOTH KAVITHA,GOPI SWAPNA,PITTA LAVANYA. "Novel Anlytical Rp-Hplc Method for Simultaneous Determination of Antiretroviral Drugs." International Journal of Pharmacy Research & Technology Online First: 23 Sep, 2022. doi:10.31838/ijprt/13.01.11


MLA (The Modern Language Association) Style

USHA KONDLA, VANKUDOTH KAVITHA,GOPI SWAPNA,PITTA LAVANYA. "Novel Anlytical Rp-Hplc Method for Simultaneous Determination of Antiretroviral Drugs." International Journal of Pharmacy Research & Technology Online First: 23 Sep, 2022. Web. 03 Mar 2023 doi:10.31838/ijprt/13.01.11


APA (American Psychological Association) Style

USHA KONDLA, VANKUDOTH KAVITHA,GOPI SWAPNA,PITTA LAVANYA (2022) Novel Anlytical Rp-Hplc Method for Simultaneous Determination of Antiretroviral Drugs. International Journal of Pharmacy Research & Technology, Online First: 23 Sep, 2022. doi:10.31838/ijprt/13.01.11




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