Evaluation Of Antioxidant and Anti-Inflammatory Activity of Blumea Moliis

Abstract

Objective: Objective of the present study was to carry out in vivo anti-inflammatory and in vitro antioxidant activity of ethanol extract of aerial part of the Blumea moliis belonging to family Asteraceae.
Methods: The shade dried aerial part of B. moliis (0.5 kg) was powdered successively extracted with ethanol (1.5 x 3L) at room temperature (24h x 3). After filtration combined all the three extracts and were concentrated on rotary evaporator under reduced pressure at 40 °C, thereby providing crude ethanol extract which was subsequently employed for further studies. Anti-inflammatory effect was studied by carrageenan- induced paw edema model in rats at dose level 100, 200, and 400 mg/kg. Acute oral toxicity study and in vitro antioxidant potential of the extract was also studied. The in vitro antioxidant activity of ethanol extract of aerial part of Blumea moliis was evaluated against 1,1- diphenyl-2-picryl hydrazyl (DPPH), hydrogen peroxide (H2O2) and hydroxyl (OH) radical scavenging and reducing power assays.
Results: The results indicate that ethanol extract of Blumea moliis (BMEE, 400 mg/kg) exhibited significant inhibition (p<0.001) of increase in paw edema at 5th h. IC50 value of BMEE showed significant antioxidant activity. The extract exhibits promising free radical scavenging effect of DPPH, H2O2, OH and reducing power in a dose-dependent manner up to 100μg/ml concentration while the reference standard Ascorbic acid demonstrated more scavenging potential than the ethanol extract of Blumea Moliis The ethanol extract was found to be safe at the dose of 2000 mg/kg.
Conclusion: The results of the experimental study confirmed that ethanol extract of Blumea Moliis possesses significant anti-inflammatory and presence of antioxidant activity.