Uv Spectrophotometric Method For Determination Of Bifonazole

Abstract

A simple, precise, accurate and reproducible UV-spectrophotometric method has been developed and validated for quantification of Bifonazole in bulk. Bifonazole is soluble in 0.1M HCl. The UV spectrum of Bifonazole in 0.1M HCl shows maximum absorbance at 254nm after scanning in 200-400nm range. The concentration range for analysis was 0.02-0.12 µg/ml. The method was validated for linearity, accuracy, precision, robustness, LOD, LOQ and ruggedness. The linearity equation was found to be y = 11.13x + 0.0294 with correlation coefficient R² = 0.9978. LOD and LOQ were found to be 0.123612255µg/ml and 0.374582589µg/ml respectively. The %recovery was found to be in range 91-103.91%. Based on the validation results the method can be successfully used for determination of Bifonazole in bulk.