Development and Validation of UV Spectroscopic Method for Estimation of Symplocos Racemosa
Keywords:
Symplocosracemosa, UV-Spectrophotometric, Obesity, Lodhra, Marketed Formulation.Abstract
Background: Symplocos racemosa Roxb. Is an herbal plant grown in tropical and subtropical regions around the globe. Due to its therapeutic activity, it is being used as an herbal treatment against obesity and many other chronic and acute disease, considering these applications of the drug a UV-Spectrophotometric method was developed and validated according to ICH (International Council for Harmonization) recommendations.
Objective: To develop a quick, precise, reliable and specific UV-Spectrophotometric method validate according to ICH Q2 (R1) guidelines.
Materials and Method: Methanol was used as the solvent for method development at a wavelength of 276nm.
Results: The developed method demonstrated correlation coefficient value of 0.999and was found to be linear with a low LOQ and LOD values of 12.21 and 37 respectively with accuracy and precision %RSD (Relative standard Deviation) value less than 2.
Conclusion: A quick, accurate, simple and economical UV-Spectroscopy method was developed and validated for the estimation of Symplocos Racemosa.
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